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| Pages | Subpages | Contents | Status Gutenberg | |||
|---|---|---|---|---|---|---|
| Contact | Correspondence form for suggestions and questions. | Complete | ||||
| Inhibition and nonessential activation | Definition of linear and hyperbolic modifiers. The fundamental rate equation based on the general modifier mechanism by Botts and Morales (1953.) Acronyms and full names of all mechanisms. | Complete | ||||
| Essential activation | Rate equations and systematics of the four mechanisms of essential activation. Acronyms and full names of all mechanisms. | Complete | ||||
| Raw data | Technical points and practical suggestions in handling rate measurements of enzyme inhibition and activation. Step-by-step tutorial with a practical example to extract information from available data. | Complete | ||||
| Concentration-dependence of parameters | Undoubtedly the most powerful method for determining the basic enzyme inhibition and activation mechanisms. This is a necessary step before proceeding to any nonlinear regression procedure! | Complete | ||||
| The specific velocity plot | A 'dissection' method that helps determining the mechanism of inhibition or activation and the allosteric coupling constant. Fundamental for setting constraints in critical nonlinear regression procedures. | Complete | ||||
| The final polish | With the information from the two preceding steps, the straightforward way to impeccable calculations of kinetic parameters. | Complete | ||||
| Tightly-bound modifiers | A frequently overlooked situation when a modifier is tightly bound to the enzyme. A dedicated equation takes into account any depletion of free modifier and allows calculating correct parameters. | Complete | ||||
| A word on software | Brief description of useful packages in enzyme kinetics. | Complete | ||||
| Basic modifier mechanisms | Validation of analyses of the 17 basic modifier mechanisms. | Complete | ||||
| Constructive criticism | To reflect on things that people might prefer not to know about | Complete | ||||
| Plan of the website | Overview of sections and pages. Information on any planned and/or still incomplete pages. | Dynamic | ||||
| 17 pages, each one dedicated to a basic modifier mechanism | A selection of examples from several branches of biochemical/biological sciences to illustrate not only the existence of all 17 basic modifier mechanisms, but also to demonstrate the strategies used by researches to determine mechanisms and kinetic parameters. | Dynamic | ||||
| List of all examples | Searchable table containing all practical examples from the 17 preceding subsections. | Dynamic | ||||
| Enzyme inactivation (irreversible inhibition) | Tutorial: the known mechanisms and their analysis. | Planned | ||||
| Mechanism-based enzyme inactivation | Tutorial: the known mechanisms and their analysis. | Planned | ||||
| A word of advice | Just a few memos not to forget essential information when publishing. | Complete | ||||
| About | Shortly about the author of this website and a reference book. | Complete | ||||
| Data analysis | A tutorial section. Suggestions for the analysis of the basic enzyme-modifier mechanisms. Workflow diagram, graphical abstracts of the five subsections. | Complete | ||||
| Data validation | Aim of an easy-to-use validation method for the identification of enzyme-modification mechanisms. | Complete | ||||
| Introduction | Addresses the aims of this work. | Complete | ||||
| Irreversible modification | Introduction to irreversible enzyme modification. | Planned | ||||
| Mechanisms | A tutorial section. Introduction to the systematics of the 17 basic enzyme modification mechanisms. List of symbols and units. | Complete | ||||
| Published examples | Explanation of the aim of this compilation from a large bibliography. | Complete | ||||
| Slow-onset inhibition | Slow-onset enzyme inhibition | Tutorial: the known types and their analysis. | Planned |