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Pages Subpages Contents Status
IntroductionAddresses the aims of this work.Complete
Plan of the websiteOverview of sections and pages. Information on any planned and/or still incomplete pages.Dynamic
MechanismsA tutorial section. Introduction to the systematics of the 17 basic enzyme modification mechanisms.
List of symbols and units.
Complete
Inhibition and nonessential activationDefinition of linear and hyperbolic modifiers.
The fundamental rate equation based on the general modifier mechanism by Botts and Morales (1953.)
Acronyms and full names of all mechanisms.
Complete
Essential activationRate equations and systematics of the four mechanisms of essential activation.
Acronyms and full names of all mechanisms.
Complete
Data analysisA tutorial section. Suggestions for the analysis of the basic enzyme-modifier mechanisms.
Workflow diagram, graphical abstracts of the five subsections.
Complete
Raw dataTechnical points and practical suggestions in handling rate measurements of enzyme inhibition and activation.
Step-by-step tutorial with a practical example to extract information from available data.
Complete
Concentration-dependence of parametersUndoubtedly the most powerful method for determining the basic enzyme inhibition and activation mechanisms.
This is a necessary step before proceeding to any nonlinear regression procedure!
Complete
The specific velocity plotA 'dissection' method that helps determining the mechanism of inhibition or activation and the allosteric coupling constant.
Fundamental for setting constraints in critical nonlinear regression procedures.
Complete
The final polishWith the information from the two preceding steps, the straightforward way to impeccable calculations of kinetic parameters.Complete
Tightly-bound modifiersA frequently overlooked situation when a modifier is tightly bound to the enzyme. A dedicated equation takes into account any depletion of free modifier and allows calculating correct parameters.Complete
A word on softwareBrief description of useful packages in enzyme kinetics.Complete
Published examplesExplanation of the aim of this compilation from a large bibliography.Complete
17 pages, each one dedicated to a basic modifier mechanismA selection of examples from several branches of biochemical/biological sciences to illustrate not only the existence of all 17 basic modifier mechanisms, but also to demonstrate the strategies used by researches to determine mechanisms and kinetic parameters.Dynamic
List of all examplesSearchable table containing all practical examples from the 17 preceding subsections.Dynamic
Irreversible modificationIntroduction to irreversible enzyme modification.Under construction
Enzyme inactivation (irreversible inhibition)Tutorial: the known mechanisms and their analysis.Under construction
Mechanism-based enzyme inactivationTutorial: the known mechanisms and their analysis.Under construction
Slow-onset inhibitionSlow-onset enzyme inhibitionTutorial: the known types and their analysis.Under construction
Data validationAim of an easy-to-use validation method for the identification of enzyme-modification mechanisms.Complete
Basic modifier mechanismsValidation of analyses of the 17 basic modifier mechanisms.Complete
A word of adviceJust a few memos not to forget essential information when publishing.Complete
Constructive criticismTo reflect on things that people might prefer not to know aboutComplete
AboutShortly about the author of this website and a reference book.Complete
ContactCorrespondence form for suggestions and questions.Complete