Your results confirmed that, for increasing modifier concentration:
- kcat decreases as hyperbola
- 1/kcat increases as straight line
- Km decreases as hyperbola
For the final discrimination we exploit the dependence of kcat/Km on [X]. Choose one of the following two possibilities.
<em>k</em><sub>cat</sub>/<em>K</em><sub>m</sub> is independent of modifier concentration
The sought mechanism is:
Linear catalytic inhibition, LCaI
Examples of LCaI
<em>k</em><sub>cat</sub>/<em>K</em><sub>m</sub> decreases as hyperbola
The sought mechanism is:
Linear mixed, predominantly catalytic inhibition, LMx(Sp<Ca)I
Examples of LMx(Sp<Ca)I
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