Type 3: final discrimination

Your results confirmed that, for increasing modifier concentration:

  • kcat decreases as hyperbola
  • 1/kcat increases as straight line
  • Km decreases as hyperbola

For the final discrimination we exploit the dependence of kcat/Km on [X]. Choose one of the following two possibilities.

kcat/Km is independent of modifier concentration

The sought mechanism is:

Linear catalytic inhibition, LCaI

Examples of LCaI

kcat/Km decreases as hyperbola

The sought mechanism is:

Linear mixed, predominantly catalytic inhibition, LMx(Sp<Ca)I

Examples of LMx(Sp<Ca)I


Go back to the preceding decision step

Reset and start a new analysis