Your results confirmed that, for increasing modifier concentration:
- kcat decreases as hyperbola
- 1/kcat increases as straight line
- Km decreases as hyperbola
For the final discrimination we exploit the dependence of kcat/Km on [X]. Choose one of the following two possibilities.
kcat/Km is independent of modifier concentration
kcat/Km decreases as hyperbola
The sought mechanism is:
Linear mixed, predominantly catalytic inhibition, LMx(Sp<Ca)I
Go back to the preceding decision step