Your results confirmed that, for increasing modifier concentration:
- kcat decreases as hyperbola
- 1/kcat increases as straight line
- Km decreases as hyperbola
![](http://www.enzyme-modifier.ch/wp-content/uploads/2019/11/Validation-Type-3_-Km-decreases-hyperbola-1024x250.png)
For the final discrimination we exploit the dependence of kcat/Km on [X]. Choose one of the following two possibilities.
Go back to the preceding decision step