Your results confirmed that, for increasing modifier concentration:
- kcat decreases as hyperbola
- 1/kcat increases as hyperbola
- Km decreases as hyperbola
For the final discrimination we exploit the dependence of kcat/Km on [X]. Choose one of the following three possibilities.
kcat/Km is independent of modifier concentration
kcat/Km decreases as hyperbola
The sought mechanism is:
Hyperbolic mixed, predominantly catalytic inhibition, HMx(Sp<Ca)I
kcat/Km increases as hyperbola
The sought mechanism is:
Hyperbolic mixed, dual modification (activation → inhibition)
Go back to the preceding decision step