Your results confirmed that, for increasing modifier concentration:
- kcat increases as hyperbola
- 1/kcat decreases as hyperbola
- Km increases as hyperbola
For the final discrimination we exploit the dependence of kcat/Km on [X]. Choose one of the following three possibilities.
<em>k</em><sub>cat</sub>/<em>K</em><sub>m</sub> decreases as hyperbola
The sought mechanism is:
Hyperbolic mixed dual modification (inhibition→activation), HMxD(I/A)
<em>k</em><sub>cat</sub>/<em>K</em><sub>m</sub> is independent of modifier concentration
<em>k</em><sub>cat</sub>/<em>K</em><sub>m</sub> increases as hyperbola
The sought mechanism is:
Hyperbolic mixed, predominantly specific activation, HMx(Sp>Ca)A
Go back to the preceding decision step